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Schematic diagram showing the mechanistic basis for the <t>GloSensor</t> <t>Caspase</t> 3/7 reporter. In response to apoptosis, activation of Caspase 3/7 leads to cleavage of the intervening peptide DEVD in the GloSensor Caspase 3/7 reporter. This caspase-mediated cleavage of the DEVD sequence results in the reconstitution of the enzymatic activity and a resultant increase in bioluminescence in the presence of luciferin substrate (shown in purple)
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glosensor caspase 3/7  (Promega)
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Schematic diagram showing the mechanistic basis for the <t>GloSensor</t> <t>Caspase</t> 3/7 reporter. In response to apoptosis, activation of Caspase 3/7 leads to cleavage of the intervening peptide DEVD in the GloSensor Caspase 3/7 reporter. This caspase-mediated cleavage of the DEVD sequence results in the reconstitution of the enzymatic activity and a resultant increase in bioluminescence in the presence of luciferin substrate (shown in purple)
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d54-caspase-3/7 glosensor cells (200,000 cells)  (Millipore)
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Schematic diagram showing the mechanistic basis for the <t>GloSensor</t> <t>Caspase</t> 3/7 reporter. In response to apoptosis, activation of Caspase 3/7 leads to cleavage of the intervening peptide DEVD in the GloSensor Caspase 3/7 reporter. This caspase-mediated cleavage of the DEVD sequence results in the reconstitution of the enzymatic activity and a resultant increase in bioluminescence in the presence of luciferin substrate (shown in purple)
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Schematic diagram showing the mechanistic basis for the GloSensor Caspase 3/7 reporter. In response to apoptosis, activation of Caspase 3/7 leads to cleavage of the intervening peptide DEVD in the GloSensor Caspase 3/7 reporter. This caspase-mediated cleavage of the DEVD sequence results in the reconstitution of the enzymatic activity and a resultant increase in bioluminescence in the presence of luciferin substrate (shown in purple)

Journal: Proteases and Cancer

Article Title: Imaging Proteolytic Activities in Mouse Models of Cancer

doi: 10.1007/978-1-4939-7595-2_22

Figure Lengend Snippet: Schematic diagram showing the mechanistic basis for the GloSensor Caspase 3/7 reporter. In response to apoptosis, activation of Caspase 3/7 leads to cleavage of the intervening peptide DEVD in the GloSensor Caspase 3/7 reporter. This caspase-mediated cleavage of the DEVD sequence results in the reconstitution of the enzymatic activity and a resultant increase in bioluminescence in the presence of luciferin substrate (shown in purple)

Article Snippet: The GloSensor Caspase 3/7 biosensor is encoded by the pGLS-30F construct and is also commercially available (GLS Caspase 3/7, Promega).

Techniques: Activation Assay, Sequencing, Activity Assay

Schematic representation of the GloSensor Caspase 3/7 reporter . The reporter comprises of an N-terminal domain coding for the C terminus (358–544) of luciferase and C-terminal domain coding for the N terminus (4–354) of firefly luciferase with an intervening Caspase 3/7 recognition sequence DEVD

Journal: Proteases and Cancer

Article Title: Imaging Proteolytic Activities in Mouse Models of Cancer

doi: 10.1007/978-1-4939-7595-2_22

Figure Lengend Snippet: Schematic representation of the GloSensor Caspase 3/7 reporter . The reporter comprises of an N-terminal domain coding for the C terminus (358–544) of luciferase and C-terminal domain coding for the N terminus (4–354) of firefly luciferase with an intervening Caspase 3/7 recognition sequence DEVD

Article Snippet: The GloSensor Caspase 3/7 biosensor is encoded by the pGLS-30F construct and is also commercially available (GLS Caspase 3/7, Promega).

Techniques: Luciferase, Sequencing

Development of transgenic mice conditional for the Caspase 3/7 GloSensor reporter. A genetically engineered transgenic mouse was generated using an expression cassette wherein the GloSensor Caspase 3/7 reporter was preceded by a floxable GFP/stop cassette in pCLEX vector driven by CAG promoter. Reporter expression is achieved in a Cre-dependent manner which can simultaneously be detected by the loss of EGFP expression

Journal: Proteases and Cancer

Article Title: Imaging Proteolytic Activities in Mouse Models of Cancer

doi: 10.1007/978-1-4939-7595-2_22

Figure Lengend Snippet: Development of transgenic mice conditional for the Caspase 3/7 GloSensor reporter. A genetically engineered transgenic mouse was generated using an expression cassette wherein the GloSensor Caspase 3/7 reporter was preceded by a floxable GFP/stop cassette in pCLEX vector driven by CAG promoter. Reporter expression is achieved in a Cre-dependent manner which can simultaneously be detected by the loss of EGFP expression

Article Snippet: The GloSensor Caspase 3/7 biosensor is encoded by the pGLS-30F construct and is also commercially available (GLS Caspase 3/7, Promega).

Techniques: Transgenic Assay, Generated, Expressing, Plasmid Preparation